Potensi Bakteri Asal Bambu dalam Memproduksi Asam Indol Asetat (IAA)
Potensi Bakteri Asal Bambu dalam Memproduksi Asam Indol Asetat (IAA)
DOI:
https://doi.org/10.33019/agrosainstek.v5i1.233Kata Kunci:
Bambu, Bakteri endofit, Bakteri rizosfer, Produksi IAAAbstrak
Bambu dikenal sebagai memiliki kemampuan adaptif yang tinggi dalam mentoleransi perubahan ataupun cekaman lingkungan. Mikroorganisme lokal pada beberapa bagian tanaman bambu telah banyak dilaporkan dapat dimanfaatkan sebagai pupuk organik dan biokompos, namun potensi bakteri asal tanaman bambu sebagai penghasil IAA belum banyak dilaporkan. Dalam penelitian ini dilakukan isolasi bakteri rizosfer dan endofit yang berasal dari enam jenis bambu berbeda. Sampel bambu diperoleh dari kawasan hutan bambu Stasiun Mengkendek Tana Toraja. Isolat bakteri rizosfer diperoleh dari daerah sekitar perakaran tanaman bambu, sedangkan isolat bakteri endofit diperoleh dari jaringan segar akar dan rebung bambu. Dalam penelitian ini diperoleh 6 isolat bakteri rizosfer dan 12 isolat bakteri endofit. Seluruh isolat diidentifikasi bentuk sel, tipe Gram, uji biokimia, serta diukur kemampuannya dalam memproduksi IAA. Dari 18 isolat, diperoleh 12 isolat penghasil IAA. Isolat bakteri endofit mendominasi perolehan isolat penghasil IAA. Dari hasil identifikasi 16S diketahui isolat K12 memiliki kemiripan dengan Bacillus cereus dan mampu menghasilkan IAA sebesar 1.301 mg L-1, sedangkan isolat K14 memiliki kemiripan dengan Stenotrophomonas maltophilia dengan kemampuan menghasilkan IAA sebesar 2.737 mg L-1. Hasil rekonstruksi pohon filogeni menunjukkan isolat K12 memiliki kemiripan pasangan basa dengan Bacillus weidmannii, dan isolat K14 memiliki kekerabatan dengan kelompok Stenotrophomonas sp.
Unduhan
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