The Effect of 2,4-D and BAP On The Multiplication of Vanilla (Vanilla planifolia Andrews)
Pengaruh 2,4-D dan BAP terhadap Multiplikasi Tanaman Vanili (Vanilla planifolia Andrews)
DOI:
https://doi.org/10.33019/agrosainstek.v9i2.912Keywords:
Hormone, In-vitro, Multiplication, VanillaAbstract
Vanilla is a plant that has many benefits, high selling value and potential to be developed. Conventional propagation of vanilla such as through cuttings has not been effective, so a more effective method of propagation is needed, one of which is propagation through tissue culture. In-vitro propagation of vanilla can be through shoot multiplication to produce fast plantlets. One of the successes of shoot multiplication is influenced by the hormone given. This study aims to determine the effect of giving a combination of 2,4-D and BAP on the multiplication of vanilla shoots. The study design used CRD with a combination factor of 2,4-D (concentration 0.5 mg/L, 1.0 mg/L, and 1.5 mg/L) and BAP (concentration 0.25 mg/L, 0.5 mg/L, and 0.75 mg/L). The explants used were vanilla stem segments. Based on the research results, the combined treatment with concentrations of 2,4-D 1 mg/L and BAP 0.5 mg/L had a very significant effect on the multiplication of vanilla shoots with the fastest emergence of shoots with an average of 6.6 wap, the highest number of shoots was 7 shoots, and the survival percentage was 100%.
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References
Anis M, Ahmad N. 2016. Plant tissue culture: Propagation, conservation and crop improvement, Plant Tissue Culture: Propagation, Conservation and Crop Improvement. Springer. doi: 10.1007/978-981-10-1917-3.
Abebe Z, Mengesha A, Teressa A, Tefera E. 2009. Efficient in vitro multiplication protocol for Vanilla planifolia using nodal explants in Ethiopia. African Journal of Biotechnology. 8(24), pp. 6817–6821.
Chika S, Kurniawati F, Rahmani TPD. 2021. Kajian Budidaya Tanaman Anggrek Dendrobium sp. dengan Teknik Kultur Meristem serta Pengaruh Penambahan Berbagai Ekstrak terhadap Pertumbuhannya. Prosiding Biologi Achieving the Sustainable Development Goals with Biodiversity in Confronting Climate Change, (November), pp. 434–441. Available at: https://journal.uin-alauddin.ac.id/index.php/psb.
Dwitama AG, Darsono, Fajarningsih RU. 2022. Analisis Kinerja Perdagangan Dan Daya Saing Komoditas Vanili Indonesia Di Pasar Internasional Periode 2010-2019. Agrista, 10(2), 43–58.
Dwiyani R. 2015. Kultur Jaringan Tanaman. Cetakan Pertama. Denpasar Barat: Pelawa Sari.
Erawati DN, Wardati I, Humaida S, Mawadah Y, Ikanafi'ah A, Ryana WM. 2021. Shoots multiplication of vanilla (Vanilla planifolia) with benzyl amino purine and kinetin modification. IOP Conference Series: Earth and Environmental Science. 672(1), pp. 0–6. doi: 10.1088/1755-1315/672/1/012007.
Erawati DN, Fisdiana U, Kadafi M. 2020. Respon Eksplan Vanili (Vanilla planifolia) dengan Stimulasi BAP dan NAA Melalui Teknik Mikropropagasi. Agriprima : Journal of Applied Agricultural Sciences. 4(2), pp. 146–153. doi: 10.25047/agriprima.v4i2.362.
Gantait S, Kundu S. 2017. In vitro biotechnological approaches on Vanilla planifolia Andrews: advancements and opportunities. Acta Physiologiae Plantarum. 39(9), pp. 1–19. doi: 10.1007/s11738-017-2462-1.
Hernandez F, Dolce NR, Flores O, Rascon MP. 2020. Advances in cryopreservation of vanilla (Vanilla planifolia Jacks.) shoot-tips: assessment of new biotechnological and cryogenic factors. In Vitro Cellular and Developmental Biology - Plant. 56(2), pp. 236–246. doi: 10.1007/s11627-020-10069-w.
Janarthanam B, Seshadri S. 2008. Plantlet regeneration from leaf derived callus of Vanilla planifolia Andr. In Vitro Cellular and Developmental Biology - Plant. 44(2), pp. 84–89. doi: 10.1007/s11627-008-9123-4.
Karjadi A, Buchory A. 2008. Sifat Inovasi Dan Aplikasi Teknologi Pengelolaan Terpadu Kebun Jeruk Sehat Dalam Pengembangan Agribisnis Jeruk Di Kabupaten Sambas, Kalimantan Barat. Jurnal Hortikultura. 18(4), pp. 380–384.
Kumar NSS. 2014. Induction of vanillin related compounds from nodal explants of Vanilla planifolia using BAP and Kinetin. Pelagia Research Library Asian Journal of Plant Science and Research. 4(1), pp. 53–61. Available at: www.pelagiaresearchlibrary.com.
Kusbianto DE, Kurniawan NC, Arum A, Restanto DP. 2022. Respon Bap Dan 2,4-D Terhadap Induksi Tunas Tanaman Vanili (Vanilla planifolia). Jurnal Ilmu-Ilmu Pertanian Indonesia. 24(2), pp. 82–87. doi: 10.31186/jipi.24.2.82-87.
Kusbianto DE, Haliza N, Restanto DP, Wulanjari D, Avivi S, Prayoga MC. 2024. The Effect of Benzyl Amino Purine ( BAP ) and Coconut Water on the Growth of Vanilla ( Vanilla planifolia Andrews .) In Vitro. Jurnal Nature Indonesia. 22(2): 76-83. https://doi.org/10.31258/jnat/22.2.76-83
Manokari M, Priyadharshini S, Jogam P, Dey A. 2021. Meta-topolin and liquid medium mediated enhanced micropropagation via ex vitro rooting in Vanilla planifolia Jacks. ex Andrews. Plant Cell, Tissue and Organ Culture. 146(1), pp. 69–82. doi: 10.1007/s11240-021-02044-z.
Nurholis. 2017. Perbanyakan Tanaman Panili (Vanilla planifolia Andrews) Secara Setek dan Upaya untuk Mendukung Keberhasilan Serta Pertumbuhannya. Agrovigor: Jurnal Agroekoteknologi. 10(2), pp. 149–156. doi: 10.21107/agrovigor.v10i2.4242.
Palama TL, Menard P, Fock I, Choi YH, Bourdon E, Govinden J, Bahut M, Payet B, Verpoorte R, Kodja H. 2010. Shoot differentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae): proteomic and metabolic responses at early stage. BMC Plant Biology. 1-18. https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-10-82.
Pathak AR, Joshi AG. 2021. Regeneration of hemidesmus indicus (L.) r. br. using in vitro nodes: An alternative method for efficient multiplication of shoots. Notulae Scientia Biologicae. 13(2), pp. 1–8. doi: 10.15835/nsb13210831.
Prasaja D, Cahyono FK, Hanifa HAI, Nisa SK. 2024. Potensi Indonesia Menjadi Pengekspor Vanili Terbesar Di Dunia. Journal of Science and Social Research, 4307(1), 265–272. http://jurnal.goretanpena.com/index.php/JSSR
Priefert H, Rabenhorst J, Steinbuchel A. 2001. Biotechnological production of vanillin. Applied Microbiology and Biotechnology. 56(3–4), pp. 296–314. doi: 10.1007/s002530100687.
Raesita D, Timburas, Arthur G, Pinaria, Edy FL. 2023. Pengaruh Beberapa Konsentrasi Zat Pengatur Tumbuh (ZPT) Auksin Naa (Naphthalene Acetic Acid) Pada Pertumbuhan Akar Stek Vanili (Vanilla planifolia Andrews). Jurnal Agroekoteknologi Terapan Universitas Sam Ratulangi. 4(1), pp. 67–73.
Ramirez-Mosqueda MA, Bello-Bello JJ. 2021. SETISTM bioreactor increases in vitro multiplication and shoot length in vanilla (Vanilla planifolia Jacks. Ex Andrews). Acta Physiologiae Plantarum. 43(4), pp. 1–8. doi: 10.1007/s11738-021-03227-z.
Rosman R. 2015. Status dan strategi pengembangan panili di Indonesia. Balai Penelitian Tanaman Rempah dan Obat. Perspektif. 4(2), pp. 43–54.
Safitri R, Prihastanti E. 2023. Optimization of vanilla (Vanilla planifolia Andrews) growth with auxin and Trichoderma harzianum combination treatment. Journal of Applied Horticulture, 25(3), 238–242. https://doi.org/10.37855/jah.2023.v25i03.42
Soch J, Sonka J, Ponert J. 2023. Acid scarification as a potent treatment for an in vitro germination of mature endozoochorous Vanilla planifolia seeds. Botanical Studies. 64(1). doi: 10.1186/s40529-023-00374-z.
Solano MCP, Ruiz JS, Arnao MTG, Castro OC, Tovar MEG, Bello JJB. 2019. Evaluation of in vitro shoot multiplication and ISSR marker based assessment of somaclonal variants at different subcultures of vanilla (Vanilla planifolia Jacks). Physiology and Molecular Biology of Plants, 25(2), pp. 561–567. doi: 10.1007/s12298-019-00645-9.
Spinoso J, Santoscoy AC, Bogdanchikova N, Sato JAP. 2017. Antimicrobial and hormetic effects of silver nanoparticles on in vitro regeneration of vanilla (Vanilla planifolia Jacks. ex Andrews) using a temporary immersion system. Plant Cell, Tissue and Organ Culture. 129(2), pp. 195–207. doi: 10.1007/s11240-017-1169-8.
Tan BC, Chin CF, Alderson P. 2013. Effects of sodium nitroprusside on shoot multiplication and regeneration of Vanilla planifolia Andrews. In Vitro Cellular and Developmental Biology - Plant. 49(5), pp. 626–630. doi: 10.1007/s11627-013-9526-8.
Timburas RD, Pinaria AG, Lengkong EF. 2023. The Effect Of Several Concentrations Of Growth Regulatory Substance (ZPT) Auxin NAA (Naphthalene Acetic Acid) On The Root Growth Of Vanila (Vanila planifolia Andrew) Cuttings. Jurnal Agroekoteknologi Terapan, 4(1), 67–73. https://doi.org/10.35791/jat.v4i1.44100.
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