Effectivity Test of Biological Agent Metarizhium anisopliae on Cluster Caterpillar (Spodoptera litura Fabr) by In Vitro Method

  • Riwan Kusmiadi University of Bangka Belitung
  • Sitti Nurul Aini University of Bangka Belitung
  • Rion Apriyadi University of Bangka Belitung
  • Ciko Ciko University of Bangka Belitung
Keywords: M. anisopliae, Spodoptera litura, mortality

Abstract

Pests are one the reason of declined in crop production. one of them is Spodoptera litura. The purpose of this research was to determined the mortality rate of Spodoptera litura caused by M. anisopliae infection. The research is conducted at the Microbiology Laboratory of Agricultural Technology Department of the Faculty of Agriculture, Fishery, and Biology of Universitas Bangka Belitung. The research used factorial randomized block design with 2 treatment factors. The first factor ( stage larvae) consists of L1=instar 3, L2=instar 4. The second factor (the concentration) consists of V0=control, V1= 30 g/l of water, V2=35 g/l of water, V3= 40 g/l of water, V4= 45 g/l of water. The data is analyzed using ANOVA with confidence level of 95% and is further tested using the Tukey Test with the confidence level of 95%. The research results show that a concentration of 30 g/l of water and 35 g/l of water  the mortality rate reaches 88,89%, while at the concentration of 40 g/l of water and 45 g/l of water the mortality rate reaches 100% with 3 to 7 days span of mortality. The larvae infected with M. anisopliae experience behavioral changes such as decreased appetite, less active movement, inactive, and transformed color to black with mycelium covering a part of the body.

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Author Biographies

Riwan Kusmiadi, University of Bangka Belitung

Department of Agrotechnology, Faculty of Agriculture, Fisheries,
and Biology

Sitti Nurul Aini, University of Bangka Belitung

Department of Agrotechnology, Faculty of Agriculture, Fisheries,
and Biology

Rion Apriyadi, University of Bangka Belitung

Department of Agrotechnology, Faculty of Agriculture, Fisheries,
and Biology

Ciko Ciko, University of Bangka Belitung

Department of Agrotechnology, Faculty of Agriculture, Fisheries,
and Biology

Published
2017-12-15
How to Cite
Kusmiadi, R., Aini, S., Apriyadi, R. and Ciko, C. (2017) “Effectivity Test of Biological Agent Metarizhium anisopliae on Cluster Caterpillar (Spodoptera litura Fabr) by In Vitro Method”, AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian, 1(2), pp. 86-94. doi: 10.33019/agrosainstek.v1i2.12.
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