Comparative Analysis on Metabolites of Torbangun (Plectranthus amboinicus (Lour.) Spreng.) Leaves Mutant

Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.)

  • Syarifah Iis Aisyah Bogor Agriculture University
  • Henny Rusmiyati Bogor Agriculture University
  • Dewi Sukma Bogor Agriculture University
  • Rizal Damanik Bogor Agriculture University
  • Waras Nurcholis Bogor Agriculture University
Keywords: Chemometric, GC-MS, HCA, Metabolomics, Torbangun, Plectranthus amboinicus

Abstract

Torbangun (Plectranthus amboinicus) is a medicinal plant belonging to the Lamiaceae family. In Batak society, the torbangun leaves are consumed to increase breast milk production. The phytochemical variability can be improved by gamma-ray irradiation for plant breeding programs. In this work, a non-targeted metabolomics method was followed to evaluate the compounds profile of irradiated mutant and wild-type on leaves of P. amboinicus. GC-MS analyses evaluated the mutant putative (derived from gamma-ray irradiation) and wild-type plantlets. The resulting of GC-MS data were analyzed with chemometric analysis using hierarchical cluster analysis (HCA). The chemical diversity was showed in mutant and wild-type P. amboinicus. The major metabolite in plantlet wild-type were hydroxymethylfurfural (35.15%), brevifolin (7.69%), 3-Dihydro-3,5-Dihydroxy-6-Methyl-4H-Pyran-4-one (DDMP) (13.13%), stigmasterol (6.51%) and ferruginol (8.63%). However, the compound of DDMP (19.57%), neophytadiene (8.47%), linolenic acid (9.10%), ferruginol (7.61%), stigmasterol (14.14%) and gamma-sitosterol (7.08%) were identified in mutant plantlet. HCA showed diversity between wild-type and mutant plantlets, which highest content of DDMP and stigmasterol and lowest of hydroxymethylfurfural of mutant plantlet.

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Author Biographies

Syarifah Iis Aisyah, Bogor Agriculture University

Departemen Agronomi dan Hortikultura, Fakultas Pertanian

Henny Rusmiyati, Bogor Agriculture University

Departemen Agronomi dan Hortikultura, Fakultas Pertanian

Dewi Sukma, Bogor Agriculture University

Departemen Agronomi dan Hortikultura, Fakultas Pertanian

Rizal Damanik, Bogor Agriculture University

Departemen Gizi Masyarakat, Fakultas Ekologi Manusia

Waras Nurcholis, Bogor Agriculture University

Pusat Studi Biofarmaka Tropika

Departemen Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam

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Published
2020-03-12
How to Cite
Aisyah, S., Rusmiyati, H., Sukma, D., Damanik, R. and Nurcholis, W. (2020) “Comparative Analysis on Metabolites of Torbangun (Plectranthus amboinicus (Lour.) Spreng.) Leaves Mutant”, AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian, 4(1), pp. 10-16. doi: 10.33019/agrosainstek.v4i1.109.
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