Performance of Putative Mutants and Genetic Parameters of Plectranthus amboinicus (L.) through Mutation Induction With Colchicine

Authors

  • Kunto Wibisono Banten AIAT, Ministry of Agriculture https://orcid.org/0000-0002-7140-5537
  • Syarifah Iis Aisyah IPB University
  • Waras Nurcholis IPB University
  • Sri Suhesti Indonesian Center for Estate Crops Research and Development, Ministry of Agriculture

DOI:

https://doi.org/10.33019/agrosainstek.v5i2.247

Keywords:

Colchicine, Heritability, Plectranthus amboinicus, Putative mutants

Abstract

Genetic enhancement in vegetatively propagated crops can be done through mutation induction. Colchicine-induced mutation is one of the methods that can be employed to increase plant genetic diversity. This study aimed to determine the effect of colchicine on the performance and genetic parameters of MV3 generation of Plectranthus amboinicus (L.). This study was conducted at the Laboratory of Agricultural Seed Management, Plantation Research and Development Center, from June 2018 to June 2019. Nodes and shoots were used as explants. Mutation induction was performed using colchicine at concentrations of 0%, 0.02%, 0.04%, and 0.06%. Explant regeneration and subculture were done on MS0 medium. The number of plantlets yielded were 59 (0% concentration of colchicine), 60 (0.02%), 81 (0.04%), and 80 plantlets (0.06%), respectively. Results indicated that colchicine-induced mutation in an in vitro culture was able to generate high genetic diversity in both quantitative and qualitative characters of the plantlets. At the concentration of 0.04%, colchicine produced the highest frequency of putative mutants (28.4%). Genetic parameters in MV3 generation of P. amboinicus plantlets showed that five quantitative characters, i.e. plantlet height, number of leaves, number of shoots, leaf length, and number of roots had high heritability values at a concentration around the LC50 value (0.0275%).

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Author Biographies

Kunto Wibisono, Banten AIAT, Ministry of Agriculture

Department of Agronomy and Horticulture, Faculty of Agronomy, Bogor Agricultural University (IPB University), Bogor 16680, Indonesia

Banten Assessment Institute for Agricultural Technology, Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture

 

Syarifah Iis Aisyah, IPB University

Department of Agronomy and Horticulture, Faculty of Agronomy, Bogor Agricultural University (IPB University), Bogor 16680, Indonesia.

Waras Nurcholis , IPB University

Department of Biochemistry, Faculty of Mathematics and Natural Science, Bogor Agricultural University (IPB University), Bogor 16680, Indonesia

Sri Suhesti, Indonesian Center for Estate Crops Research and Development, Ministry of Agriculture

 Indonesian Center for Estate Crops Research and Development, Ministry of Agriculture

References

Abu-Qaoud H, Shtaya MJY. 2014. The effect of colchicine on adventitious shoot regeneration from cultured leaf explants of Petunia hybrida. Br Biotechnol J. 4(5):531-540.

Aguiar JJS, Sousa CPB, Araruna MKA, Silva MKN, Portelo AC, Lopes JC, Carvalho VRA, Figueredo FG, Bitu VCN, Coutinho HDM, et al. 2015. Antibacterial and modifying-antibiotic activities of the essential oils of Ocimum gratissimum L. and Plectranthus amboinicus L. Eur J Integr Med. 7(2):151–156. doi:10.1016/j.eujim.2014.10.005.

Aisyah SI, Rusmiyati H, Sukma D, Damanik R, Nurcholis W. 2020. Analisis komparatif kandungan metabolit pada daun mutan tanaman torbangun (Plectranthus amboinicus (Lour.) Spreng). Agrosainstek. 4(1):10–16. doi:10.33019/agrosainstek.v4i1.109.

Baig MMQ, Ahmad T, Hafiz IA, Abbasi NA, Tareen MJ. 2016. In vitro improvement of characters in Rosa spp. through colchicine. J Anim Plant Sci. 26(6):1666–1674.

Bhatt P, Joseph GS, Negi PS, Varadaraj MC. 2013. Chemical composition and nutraceutical potential of Indian borage (Plectranthus amboinicus) stem extract. J Chem. 2013:1–7. doi:10.1155/2013/320329.

Bhatt P, Negi PS. 2012. Antioxidant and antibacterial activities in the leaf extracts of Indian borage (Plectranthus amboinicus). FNS. 03(02):146–152. doi:10.4236/fns.2012.32022.

Brandao EM, Brandão PHDM, Souza IA, Paiva GS, de C. Carvalho M, Lacerda CM. 2013. Antineoplasic effect of aqueous extract of Plectranthus amboinicus in Ehrlich ascites carcinoma. J Cancer. 4(7):573–576. doi:10.7150/jca.6730.

Chen Y-S, Yu H-M, Shie J-J, Cheng T-JR, Wu C-Y, Fang J-M, Wong C-H. 2014. Chemical constituents of Plectranthus amboinicus and the synthetic analogs possessing anti-inflammatory activity. Bioorg Med Chem. 22(5):1766–1772. doi:10.1016/j.bmc.2014.01.009.

Damanik RM, Kustiyah L, Hanafi M, Iwansyah AC. 2017. Evaluation lactogenic activity of ethyl acetate fraction of torbangun (Coleus amboinicus L.) leaves. IOP Conf Ser: Earth Environ Sci. 101:012007. doi:10.1088/1755-1315/101/1/012007.

Dhooghe E, Denis S, Eeckhaut T, Reheul D, Van Labeke M-C. 2009. In vitro induction of tetraploids in ornamental Ranunculus. Euphytica. 168(1):33–40. doi:10.1007/s10681-008-9876-1.

Dirar AI, Alsaadi DHM, Wada M, Mohamed MA, Watanabe T, Devkota HP. 2019. Effects of extraction solvents on total phenolic and flavonoid contents and biological activities of extracts from Sudanese medicinal plants. S Afr J Bot. 120:261–267. doi:10.1016/j.sajb.2018.07.003.

El-Latif FMA, El-Gioushy SF, Islam SE, Zakry TA. 2018. Impact of papaya seed soaking in different BA, colchicine and EMS solutions on germination, growth and chromosomal behaviour. Asian J Biotechnol Genet Eng. 1(1):1–17. doi:10.9734/AJBGE/2018/40538.

Feng H, Wang M, Cong R, Dai S. 2017. Colchicine- and trifluralin-mediated polyploidization of Rosa multiflora Thunb. var. inermis and Rosa roxburghii f. normalis. J Hortic Sci Biotechnol. 92(3):279–287. doi:10.1080/14620316.2016.1249964.

Gaafar RM, El Shanshoury AR, El Hisseiwy AA, AbdAlhak MA, Omar AF, Abd El Wahab MM, Nofal RS. 2017. Induction of apomixis and fixation of heterosis in Egyptian rice Hybrid1 line using colchicine mutagenesis. Ann Agric Sci. 62(1):51–60. doi:10.1016/j.aoas.2017.03.001.

Govindaraju S, Arulselvi PI. 2018. Characterization of Coleus aromaticus essential oil and its major constituent carvacrol for in vitro antidiabetic and antiproliferative activities. J Herbs Spices Med Plants. 24(1):37–51. doi:10.1080/10496475.2017.1369483.

Hullatti K, Bhattacharjee P. 2011. Pharmacognostical evaluation of different parts of Coleus amboinicus Lour., Lamiaceae. Pharmacogn J. 3(24):39–44. doi:10.5530/pj.2011.24.8.

Kerdsuwan N, Te-chato S. 2012. Effects of colchicine on survival rate, morphological, physiological and cytological characters of chang daeng orchid (Rhynchostylis gigantean var. rubrum Sagarik) in vitro. J Agric Sci Technol 8(4):1451-1460.

Krzywinski M, Altman N. 2014. Visualizing samples with box plots. Nat Methods. 11(2):119–120. doi:10.1038/nmeth.2813.

Kumari BP, Sujatha D, Chand CG, Divya K, Malleswari I, Ranganayakulu D. 2012. Evaluation of antiepileptic activity and probable mechanism of action of Coleus amboinicus in MES and PTZ models. J Pharm Res. 5(3):1587-1591.

Kushwah KS, Verma RC, Patel S, Jain N. 2018. Colchicine induced polyploidy in Chrysanthemum carinatum L. J Phylogenetics Evol Biol. 06(01). doi:10.4172/2329-9002.1000193. [accessed 2021 Mar 6]. https://www.omicsonline.org/open-access/colchicine-induced-polyploidy-in-chrysanthemum-carinatum-l-2329-9002-1000193-99146.html.

Manzoor A, Ahmad T, Bashir M, Hafiz I, Silvestri C. 2019. Studies on colchicine induced chromosome doubling for enhancement of quality traits in ornamental plants. Plants. 8(7):194. doi:10.3390/plants8070194.

Montalban IA, De Diego N, Moncalean P. 2011. Testing novel cytokinins for improved in vitro adventitious shoots formation and subsequent ex vitro performance in Pinus radiata. Forestry. 84(4):363–373. doi:10.1093/forestry/cpr022.

Nehra NS, Kartha KK, Stushnott C, Giles KL. 1992. The influence of plant growth regulator concentrations and callus age on somaclonal variation in callus culture regenerants of strawberry. Plant Cell Tiss Organ Cult. 29(3):257–268. doi:10.1007/BF00034361.

Ritonga AW, Chozin MA, Syukur M, Maharijaya A, Sobir S. 2018. Short communication: Genetic variability, heritability, correlation, and path analysis in tomato (Solanum lycopersicum) under shading condition. Biodiversitas. 19(4):1527–1531. doi:10.13057/biodiv/d190445.

Sadhukhan R, Ganguly A, Singh PK, Sarkar HK. 2014. Study of induced polyploidy in African marigold (Tagetes crecta L.). Environ Ecol. 32(4):1219–1222.

Sari DN, Aisyah SI, Damanik DMRM. 2017. Sensitivitas dan keragaan tanaman Coleus sp. terhadap mutasi induksi kimia menggunakan Ethyl Methane Sulfonate (EMS) aplikasi cara rendam dan tetes. J Agron Indonesia. 45(1):56–63. doi:10.24831/jai.v45i1.13157.

Saryoko A, Fukuda Y, Lubis I, Homma K, Shiraiwa T. 2018. Physiological activity and biomass production in crop canopy under a tropical environment in soybean cultivars with temperate and tropical origins. Field Crops Res. 216:209–216. doi:10.1016/j.fcr.2017.11.012.

Selvakumar P, naveena BE, prakash SD. 2012. Studies on the antidandruff activity of the essential oil of Coleus amboinicus and Eucalyptus globulus. Asian Pac J Trop Dis. 2:S715–S719. doi:10.1016/S2222-1808(12)60250-3.

Shubha JR, Bhatt P. 2015. Plectranthus amboinicus leaves stimulate growth of probiotic L. plantarum: evidence for ethnobotanical use in diarrhea. J Ethnopharmacol. 166:220–227. doi:10.1016/j.jep.2015.02.055.

Suresh S, Muthukrishnan L, Vennila S, K. G, J. AL, Paiman S, Faruq M, Al-Lohedan HA, Akbarzadeh O, Oh WC. 2020. Mechanistic anticarcinogenic efficacy of phytofabricated gold nanoparticles on human lung adenocarcinoma cells. J Exp Nanosci. 15(1):160–173. doi:10.1080/17458080.2020.1761014.

Wang D, Xue W, Ren X, Xu Z. 2021. A review on sensing mechanisms and strategies for telomerase activity detection. Trends Anal Chem. 134:116115. doi:10.1016/j.trac.2020.116115.

Wibisono K, Aisyah SI, Suhesti S, Nurcholis W. 2019. Optimization of total flavonoids extraction and Α-glucosidase inhibitory activity from Plectranthus amboinicus (Lour.) Spreng. leaves using the simplex-centroid design. Molekul. 14(2):84. doi:10.20884/1.jm.2019.14.2.497.

Yan H-J, Xiong Y, Zhang H-Y, He M-L. 2016. In vitro induction and morphological characteristics of octoploid plants in Pogostemon cablin. Breed Sci. 66(2):169–174. doi:10.1270/jsbbs.66.169.

Zhang B, Wijesundara NM, Abbey, Lord, Rupasinghe HPV. 2017. Growing medium amendments effect on growth, secondary metabolites and anti-streptococcal activity of two species of Plectranthus. J Appl Res Med Aroma. 5:53–59. doi:10.1016/j.jarmap.2016.11.001.

Zhang Y-S, Chen J-J, Cao Y-M, Duan J-X, Cai X-D. 2020. Induction of tetraploids in ‘Red Flash’ caladium using colchicine and oryzalin: morphological, cytological, photosynthetic and chilling tolerance analysis. Sci Hortic. 272:109524. doi:10.1016/j.scienta.2020.109524.

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Published

2021-12-31

How to Cite

Wibisono, K., Aisyah, S. I. ., Nurcholis , W. . and Suhesti, S. . (2021) “Performance of Putative Mutants and Genetic Parameters of Plectranthus amboinicus (L.) through Mutation Induction With Colchicine”, AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian, 5(2), pp. 89–99. doi: 10.33019/agrosainstek.v5i2.247.