The Potential of Bacteria from Bamboo in Producing Indole Acetic Acid (IAA)
Potensi Bakteri Asal Bambu dalam Memproduksi Asam Indol Asetat (IAA)
DOI:
https://doi.org/10.33019/agrosainstek.v5i1.233Keywords:
Bamboo, Endophytic bacteria, IAA production, Rhizospere bacteriaAbstract
Bamboo are known having a high adaptive ability to tolerate environmental changes or stresses. Endogenous microorganisms in several parts of bamboo have been reported used as organic fertilizer and biocompost. However, bacterial potential as auxin (IAA) producer has not been widely report, especially for Torajas’ local bamboo. In this study, rhizosphere and endophytic bacteria were isolated from six different bamboo. Bamboo samples were obtained from the bamboo forest station area of North Toraja. Rhizosphere bacterial isolates were obtained from the area around the roots of bamboo plants, while endophytic bacterial isolates were obtained from roots and shoots bamboo tissue. Six rhizosphere isolates and 12 endophytic isolates were obtaind. All isolates were indentified for morphological, physiological, biochemical tests and IAA’s activities. There are 12 IAA-producing isolates, which where dominated by endophytic bacterial isolates. Based on 16S molecular identification, it was found that K12 isolates were similar to Bacillus cereus, with an IAA concentration value was 1.301 mg L-1. While K14 isolated has similiarities with Stenotrophomonas maltophilia with the abiliy to produce IAA was 2.737 mg L-1. The reconstruction of the phylogeny tree showed that K12 isolate had similiarity with Bacillus wedimannii, and K14 isolate was related to Stenotrophomonas sp.
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References
Afzal I, Shinwari ZK, Sikandar S, Shahzad S. 2019. Plant Beneficial Endophytic Bacteria: Mechanisms, Diversity, Host Range and Genetic Determinants. Microbiological Research 221: 36-49.
Ahmed A, Hasnain S. 2010. Auxin-Producing Bacillus sp: Auxin Quantification and Effect on The Growth of Solanum tuberosum. Pure and Applied Chemistry 82(1): 313-319.
Ali H, Kermelita D. 2018. Efektifitas Mikroorganisme Lokal (MOL) Rebung Bambu Sebagai Aktivator Pembuatan Kompos Tahun 2014. Journal of Nursing and Public Health 6(1): 8-14.
Asgher M, Khan MIR, Anjum NA, Khan NA. 2015. Minimising Toxicity of Cadmium in Plants-Role of Plant Growth Regulator. Protoplasma 252: 399-413.
Aziz K, Nawaz M, Nazir J, Anjum AA, Yaqub T, Ahmad MUD, Rehman MU, Aziz G, Khan M. 2015. Isolation, Characterization and Effect of Auxin Producing Bacteria on Growth of Triticum aestivum. The Journal of Animal and Plant Sciences 25(4): 1003-1007.
Chagas Junior AF, De Oliveira AG, De Oliveira LA, Dos Santos GR, Chagas LFB, Lopes da Silva AL, Da Luz Costa J. 2015. Production of Indole-3-Acetic Acid by Bacillus Isolated From Different Soils. Bulgarian Journal of Agricultural Science 21(2), 282–287.
Chandra S, Askari K, Kumari M. 2018. Optimization of Indole Acetic Acid Production by Isolated Bacteria from Stevia rebaudiana Rhizosphere and Its Effects on Plant Growth. Journal of Genetic Engineering and Biotechnology 16(2): 581–586.
Egamberdieva D, Wirth SJ, Alqarawi AA, Abdullah EF, Hashem A. 2017. Phytohormones and Beneficial Microbes: Essential Components for Plants to Balance Stress and Fitness. Frontier in Microbiology 8: 2104(1)-2104(14).
Gustomi G, Nurusman L, Susilo S. 2018. Pengaruh Pemberian Mikroorganisme Lokal (MOL) Rebung Bambu Surat (Gigantochloa vesticillata (Willd.) Munro) Terhadap Pertumbuhan Bayam Merah (Amaranthus tricolor L.). Bioeduscience 2(1): 81-87.
Ikram R, Ali B. 2018. Co-inoculation of Auxin Producing PGPR and Rhizobia Enhanced Growth of Vigna mungo (L.) Under Cadmium Stress. Future of Food: Journal on Food, Agriculture and Society 6(1): 46–54.
Kafrawi, Nildayanti, Zahraeni K, Baharuddin. 2017. Comparison of IAA Production by Shallot Rhizosphere Isolated Bacteria in Solid and Liquid Media and Their Effect on Shallot Plant Growth. Journal of Microbial and Biochemical Technology 09(06): 266–269.
Kandel Syam L, Pierre M. Joubert, Sharon L. Doty. 2017. Bacterial Endophyte Colonization and Distribution within Plants. Microorganisms 5(77): 2-26.
Li M, Rui Go, Fei Yu, Xu Chen, Haiyan Zhao, Huixin Li, Jun Wu. 2018. Indole-3-Acetic Acid Biosynthesis Pathways in the Plant-Beneficial Bacterium Arthrobacter pascens ZZ21. International Journal of Molecular Sciences 19: 443(1)-443(15).
Mishra G, Krishna G, Shalish P, Rajesh K, N.S Bisht. 2014. Bamboo: potential resource for eco-restoration of degraded lands. Journal of Biology and Earth Sciences 4(2): B130-B136.
Pastor N, Carlier E, Andrés J, Rosas SB, Rovera M. 2012. Characterization of Rhizosphere Bacteria For Control of Phytopathogenic Fungi of Tomato. Journal of Environmental Management 95: S332-S337.
Patel MV, Patel RK. 2014. Indole-3-Acetic Acid (IAA) Production By Endophytic Bacteria Isolated From Saline Dessert, the Little Runn of Kutch. CIBTech Journal of Microbiology 3(2): 2319–3867.
Patel T, Saraf M. 2017. Exploration of Novel Plant Growth Promoting Bacteria Stenotrophomonas maltophilia MTP42 Isolated from Rhizosphere Soil of Coleus forskohlii. International Joural of Current Microbiology and Applied Science 6(11): 944-955.
Patten CL, Blakney AJC, Coulson TJD. 2013. Activity, Distribution and Function of Indole-3-Acetic Acid Biosynthetic Pathways in Bacteria. Critical Reviews in Microbiology 39(4): 395–415.
Pii Y, Mimmo T, Tomasi N, Terzano R, Cesco S, Crecchio C. 2015. Microbial Interactions in The Rizosphere: Beneficial Influence of Plant Growth-Promoting Rhizobacteria on Nutrient Acquisition Process. A review. Biology and Fertility of Soils 51(4): 403-415.
Rania ABA, Jabnoun-Khiareddine H, Nefzi A, Mokni-Tlili S, Daami-Remadi M. 2016. Endophytic Bacteria from Datura metel for Plant Growth Promotion and Bioprotection Against Fusarium Wilt in Tomato. Biocontrol Science and Technology 26(8), 1139–1165.
Spaepen S, Vanderlayden J. 2011. Auxin and Plant-Microbe Interaction. Cold Spring Harbor Perspective in Biology 3(4): a001438.
Susilowati DN, Riyanti EI, Setyowati M, Mulya K. 2018. Indole-3-Acetic Acid Producing Bacteria and Its Application on The Growth of Rice. AIP Conference Proceedings 2002 : 020016-1 – 020016-9.
Tassadaq H, Aneela R, Shehzad M, Iftikhar A, Jafar K. 2013. Biochemical Characterization and Identification of Bacterial Strains Isolated from Drinking Water Source of Kohat, Pakistan. African Journal of Microbiology Research 7(16): 1579-1590.
Wagi S, Ahmed A. 2019. Bacillus spp: Potent Microfactories of Bacterial IAA. PeerJ 7: e72589(1)-e72589(14).
Waheeda P, Radziah Othman, Hawa Jaafar, Mahbubur Rahman, Wong Mul Yun. 2015. Chromatographic detection of phytohormones from the bacterial strain UPMP3 of Pseudomonas aeruginosa and UPMB3 of Burkholderia cepacia and their role in oil palm seedling growth. International Journal of Biotechnology Research 3(5): 073-080.
Yuan ZS, Liu F, Zhang GF. 2015. Isolation of Culturable Endophytic Bacteria From Moso Bamboo (Phyllostachys edulis) and 16S rDNA Diversity Analysis. Archives of Biological Sciences 67(3): 1001–1008.
